KMID : 0364820130490030245
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Korean Journal of Microbiology 2013 Volume.49 No. 3 p.245 ~ p.252
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Probiotics with Antimicrobial Activity against Multidrug Resistant Pseudomonas aeruginosa and Acinetobacter baumannii
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Lee Do-Kyung
Kim Min-Ji Kang Joo-Yeon Park Jae-Eun Shin Hea-Soon Ha Nam-Joo
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Abstract
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Pseudomonas aeruginosa and Acinetobacter baumannii are significant opportunistic pathogens in hospitals and are
resistant to most antibiotics. Multidrug-resistant P. aeruginosa (MDRPA) and A. baumannii (MDRAB) cause severe
human nosocomial infections and are more difficult to treat than methicillin-resistant Staphylococcus aureus
(MRSA). Bifidobacteria are among of the most beneficial probiotics and have been widely studied for their
antimicrobial activities. The present study explored the antimicrobial activity of Bifidobacterium sp. isolated from
healthy Koreans against MDRPA and MDRAB. The antimicrobial activity of the isolates against MDRPA and
MDRAB, which are resistant to ciprofloxacin, tobramycin, gentamicin, meropenem, and ceftazidime, was
determined by modified broth microdilution methods using absorbance. Among all tested bifidobacteria isolates
(nine B. adolescentis, three B. longum, and two B. pseudocatenulatum), the culture supernatant of B. pseudocatenulatum
SPM1309 showed a strong growth inhibitory effect against MDRPA and MDRAB. No change in the turbidity of the
mixture was observed during incubation, and its inhibitory effect occurred through bacteriostastic action. Moreover,
the antibacterial activity was observed in the fraction with molecular weights <10 kDa of bifidobacteria culture
supernatant, and the active fraction was heat-stable because it maintained its activity when heated at 70¡É for 10
min. The results suggest that this Bifidobacterium strain could have potential applications for alternative therapy in
MDRPA and MDRAB infections.
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KEYWORD
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Bifidobacterium, antimicrobial activity, multidrug-resistant Acinetobacter baumannii, multidrug-resistant Pseudomonas aeruginosa
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